A count-dependent filter for smoothing flow cytometric histograms
نویسندگان
چکیده
منابع مشابه
A sharpness dependent filter for mesh smoothing
In this paper, we propose a sharpness dependent filter design based on the fairing of surface normal, whereby the filtering algorithm automatically selects a filter. This may be a mean-filter, a min-filter, or a filter ranked between these two, depending on the local sharpness value and the sharpness dependent weighting function selected. To recover the original shape of a noisy model, the algo...
متن کاملQuadratic form: a robust metric for quantitative comparison of flow cytometric histograms.
Comparison of fluorescence distributions is a fundamental part of the analysis of flow cytometric data. This approach is applied to detect differences between control and test sample and thus analyze a biological response. Comparison of standard test samples over time provides an estimate of instrument stability for quality control. However, application of statistical methods of distribution co...
متن کاملA multiscale polynomial filter for adaptive smoothing
The effectiveness of Savitzky–Golay type symmetric polynomial smoothers is known to be strongly dependent on the window size. Many authors note that selection of the appropriate window size is essential for achieving the correct trade-off between noise reduction and avoiding the introduction of bias. However, it is often overlooked that, in the case of non-stationary signals, the optimal window...
متن کاملSpectral analysis of flow cytometric data: design of a special-purpose low-pass digital filter.
Spectral decomposition of flow cytometric datafiles of arbitrary dimension reveal information of both the signal and the noise components that constitute the histograms. This spectral information is used to construct a low-pass digital filter, which removes the high-frequency noise from the actual data. It is shown that this procedure guarantees non-trivial smoothing of the flow cytometric data...
متن کاملFlow Cytometric Assessment of Erythrocyte Shape through Analysis of FSC Histograms: Use of Kurtosis and Implications for Longitudinal Evaluation
Sphericity of erythrocytes can be estimated from analysis of FSC signal distribution in flow cytometry. Previously, Pearson's coefficient of dissymmetry (PCD) and spherical index (SphI) were applied to determine erythrocyte sphericity from the FSC histogram. The aim of the present study is to illustrate the application of kurtosis as an indicator of erythrocyte sphericity in flow cytometry in a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry
سال: 1984
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990050509